CA9 Antibody
Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/CA9/gp200) . Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, CA9 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, CA9 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This mAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Q16790
Host
Mouse
Reactivity
Human
Immunogen
The microsomal fraction of human renal cortical tissue homogenate was used as the immunogen for this CA9 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
SPM314
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the CA9 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CA9 antibody is available for research use only.
Storage Conditions
Store the CA9 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Location
Cell surface and cytoplasmic
Image Legend
IHC: Formalin-fixed, paraffin-embedded human renal cell carcinoma stained with CA9 antibody (SPM314) .
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