Anti-UchL1 Antibody
This mAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1) . Initially, UchL1 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for UchL1 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in the gene is believed to cause a form of Parkinson's disease.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL
UniProt
P09936
Host
Mouse
Reactivity
Human
Immunogen
Native UchL1/PGP9.5 protein isolated from human brain was used as the immunogen for this anti-UchL1 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
SPM575
Applications
WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the anti-UchL1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This anti-UchL1 antibody is available for research use only.
Storage Conditions
Store the anti-UchL1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The optimal dilution of the anti-UchL1 antibody for each application should be determined by the researcher.
Location
Cytoplasmic. Endoplasmic Reticulum membrane
Image Legend
Western blot testing of human brain lysate with anti-UchL1 antibody (clone SPM575) . Predicted molecular weight ~25 kDa.
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