Anti-Thymidylate Synthase Antibody
It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) . TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P04818
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for this anti-Thymidylate Synthase antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SPM453
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the anti-Thymidylate Synthase antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This anti-Thymidylate Synthase antibody is available for research use only.
Storage Conditions
Store the anti-Thymidylate Synthase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic, nuclear
Image Legend
IHC: Formalin-fixed, paraffin-embedded human testicular carcinoma stained with anti-Thymidylate Synthase antibody (clone SPM453) .
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