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Anti-p53 Antibody

P53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P04637

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human wild type p53 protein was used as the immunogen for this anti-p53 antibody. Its epitope maps within the N-terminus (approx. amino acids 20-25) .

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

SPM589

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the anti-p53 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This anti-p53 antibody is available for research use only.

Storage Conditions

Store the anti-p53 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Nuclear

Image Legend

Western blot testing of human HeLa cell lysate with anti-p53 antibody (clone SPM589) . Expetected molecular weight ~53 kDa.

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