NKX2.1 Antibody / TTF-1
Recognizes a protein of 40kDa, identified as NKX2.1 or Thyroid transcription factor-1 (TTF-1) . TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-NKX2.1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of NKX2.1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. Reactivity is also seen in thyroid malignancies.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P43699
Host
Mouse
Reactivity
Human
Immunogen
Full length rat recombinant protein was used as the immunogen for this NKX2.1 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SPM150
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the NKX2.1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This NKX2.1 antibody is available for research use only.
Storage Conditions
Store the NKX2.1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Nuclear
Image Legend
IHC: Formalin-fixed, paraffin-embedded human lung carcinoma stained with NKX2.1 antibody (clone SPM150) .
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