CD43 Antibody
It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
Product Specifications
CAS Number
9007-83-4
Specifications
UniProt
P16150
Host
Mouse
Reactivity
Human
Immunogen
Myeloblastic KG1 cells were used as the immunogen for this CD43 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SPM503
Applications
FACS, IF, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the CD43 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD43 antibody is available for research use only.
Storage Conditions
Store the CD43 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cell surface
Image Legend
Immunofluorescence staining of human K562 cells with CD43 antibody (green, clone SPM503) and Nucspot nuclear stain (red) .
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
Human Damage Specific DNA Binding Protein 2, DDB2 ELISA KIT
Smooth muscle Myosin heavy chain 11 Recombinant Antibody, AbBy Fluor™ 555 Conjugated