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Gp100 Antibody / PMEL17

The gp100 molecule is a 100kDa glycosylated protein that is cleaved into a small (26kDa) carboxy-terminal fragment and a larger amino- terminal section (60-64 kDa), which is subsequently cleaved to generate 26kDa and 34-38kDa fragments.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P40967

Host

Mouse

Reactivity

Human

Immunogen

Extract of pigmented melanoma metastases from lymph nodes was used as the immunogen for this gp100 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

SPM142

Applications

IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the gp100 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This gp100 antibody is available for research use only.

Storage Conditions

Store the gp100 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic

Image Legend

IHC: Formalin-fixed, paraffin-embedded human melanoma stained with gp100 antibody (SPM142) .

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