Smooth Muscle Actin Antibody

Actin is a major component of the cytoskeleton and is present in most cell types. This mAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This mAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P62736

Host

Mouse

Reactivity

Human

Immunogen

A 10 amino acid peptide from the alpha smooth muscle isoform of actin was used as the immunogen for this Smooth Muscle Actin antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

SPM332

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the Smooth Muscle Actin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Smooth Muscle Actin antibody is available for research use only.

Storage Conditions

Store the Smooth Muscle Actin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cytoplasmic

Image Legend

IHC: Formalin-fixed, paraffin-embedded Leiomyosarcoma stained with Smooth Muscle Actin antibody (clone SPM332) .

Documents

Product Datasheet

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