Anti-Bcl-X Antibody
Recognizes a protein of 27kDa, identified as the Bcl-X protein. This mAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This mAb reacts with both Bcl-XS and Bcl-XL proteins.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Q07817
Host
Mouse
Reactivity
Human
Immunogen
Amino acids 3-14 (QSNRELVVDFLS) from the human protein were used as the immunogen for this anti-Bcl-X antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
SPM165
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the anti-Bcl-X antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This anti-Bcl-X antibody is available for research use only.
Storage Conditions
Store the anti-Bcl-X antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Product Datasheet
https://www.nsjbio.com/tds-pdf/anti-bcl-x-antibody-spm165-v9075
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic and cell/nuclear membrane
Image Legend
IHC: Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with anti-Bcl-X antibody (SPM165) .
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