CCND1 Antibody
Cyclin D1 (CCND1), one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. About 60% of mantle cell lymphomas (MCL) contain a t (11; 14) (q13; q32) translocation resulting in over-expression of Cyclin D1. This antibody is useful in identifying mantle cell lymphomas (CCND1+) from CLL/SLL and follicular lymphomas (CCND1-) . Occasionally, hairy cell leukemia and plasma cell myeloma weakly express CCND1.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P24385
Host
Mouse
Reactivity
Human, Mouse
Immunogen
Recombinant full length human protein was used as the immunogen for this CCND1 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
SPM587
Applications
WB, IHC-P, FACS
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the CCND1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CCND1 antibody is available for research use only.
Storage Conditions
Store the CCND1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Nuclear
Image Legend
IHC: Formalin-fixed, paraffin-embedded human mantle cell lymphoma stained with CCND1 antibody.
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