ANO1 Antibody / DOG1 / TMEM16A
Expression of DOG-1/ANO1/TMEM16A protein is elevated in the gastrointestinal stromal tumors (GISTs), c-kit signaling-driven mesenchymal tumors of the GI tract. ANO1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity has been reported in 97.8 percent of scorable GISTs, including all c-kit negative GISTs. Overexpression has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of DOG-1/ANO1 and c-kit in GISTs is nearly identical: 94.4% vs. 94.7%.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Q5XXA6
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human DOG1 protein was used as the immunogen for this ANO1 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SPM580
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the ANO1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This ANO1 antibody is available for research use only.
Storage Conditions
Store the ANO1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cell Surface and Cytoplasm
Image Legend
IHC: Formalin-fixed, paraffin-embedded human GIST stained with ANO1 antibody (clone SPM580) .
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