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Anti-PCNA Antibody

Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA) . It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases.

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P12004

Host

Mouse

Reactivity

Human

Immunogen

Rat PCNA protein was used as the immunogen for this anti-PCNA antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

SPM350

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the anti-PCNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This anti-PCNA antibody is available for research use only.

Storage Conditions

Store the anti-PCNA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Predominantly nuclear, some cytoplasmic

Image Legend

IHC analysis of formalin-fixed, paraffin-embedded human tonsil stained with anti-PCNA antibody (clone SPM350) .

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