EMA Antibody / MUC1 / Mucin-1
Epithelial Marker Antigen is a 265-400kDa glycoprotein. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P15941
Host
Mouse
Reactivity
Human
Immunogen
Human milk fat globule membranes were used as the immunogen for this EMA antibody. This mAb reacts with the DTRP epitope in the tandem repeats.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SPM132
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the EMA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This EMA antibody is available for research use only.
Storage Conditions
Store the EMA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic and cell surface
Image Legend
IHC: Formalin-fixed, paraffin-embedded human breast cancer stained with EMA antibody (clone SPM132) .
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