Fibronectin Antibody

Fibronectin is a soluble dimeric glycoprotein of 440kDa, which is present in cells, extracellular matrix, and blood. This mAb reacts with the cellular as well as plasma form of fibronectin. Reportedly, after iv administration, this mAb localizes to tumor vessels where it binds to the underlying basement. Epitope recognized by this antibody is not accessible in normal tissues to the circulating mAb indicating that it can be used to specifically target tumor vessels in vivo.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P02751

Host

Mouse

Reactivity

Human

Immunogen

A T-lymphoma biopsy was used as the immunogen for this Fibronectin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

SPM246

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the Fibronectin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Fibronectin antibody is available for research use only.

Storage Conditions

Store the Fibronectin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

The optimal dilution of the Fibronectin antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9 for 10-20 min followed by cooling at RT for 20 minutes.