Human Transforming growth factor-alpha / TGF-alpha Protein, His Tag, premium grade

Human TGF-alpha Protein, His Tag, premium grade (TGA-H5141) is expressed from E. coli cells. It contains AA Val 40 - Ala 89 (Accession # P01135-1).

Product Specifications

Background

TGF-alpha is a normal physiological ligand for the EGF receptor. TGF-α and the EGF receptor are expressed in many developing and adult tissues, where TGF-α has been implicated in cell migration, growth, and differentiation.

Specifications

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 7.4 kDa. The protein migrates as 11 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

Expression Region

Val 40 - Ala 89

Host

E. coli

Target

TGF-alpha

Conjugation

Unconjugated

Tag

N-10×His

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.01 EU per μg

Purity

90%

Bioactivity

Immobilized Human TGF-alpha Protein, His Tag, premium grade (Cat. No. TGA-H5141) at 5 μg/mL (100 μL/well) can bind Human EGF R, Fc Tag (Cat. No. EGR-H5252) with a linear range of 0.2-2 ng/mL (QC tested).