TCF4 Antibody

Recognizes a protein of 71kDa, identified as Transcription Factor 4 (TCF4) . It is a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box () - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (For coating, order BSA-free format), Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P15884

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 365-671 was used as the immunogen for the TCF4 antibody.

Clonality

Monoclonal

Isotype

IgG2c κ

Clone

TCF4/2252

Applications

ELISA, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the TCF4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This TCF4 antibody is available for research use only.

Storage Conditions

Aliquot the TCF4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the TCF4 antibody should be determined by the researcher.

Location

Nucleus, Cytoplasm

Image Legend

IHC staining of FFPE human breast carcinoma tissue with TCF4 antibody (clone TCF4/2252) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.