Recombinant Topoisomerase II alpha Antibody / TOP2A

Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and re-ligating the broken strand. [UniProt]

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 0.5-1 µg/mL

UniProt

P11388

Host

Mouse

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 1352-1493 was used as the immunogen for the recombinant Topoisomerase II alpha antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RTOP2A/6629

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant Topoisomerase II alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant Topoisomerase II alpha antibody is available for research use only.

Storage Conditions

Aliquot the recombinant Topoisomerase II alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Topoisomerase II alpha antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human lymph node with recombinant Topoisomerase II alpha antibody (clone rTOP2A/6629) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.