SOX9 Antibody

Sox genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. SOX9 plays an important role in the normal skeletal development. It may regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P48436

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human full-length SOX9 protein was used as the immunogen for the SOX9 antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

PCRP-SOX9-1A2

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the SOX9 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SOX9 antibody is available for research use only.

Storage Conditions

Aliquot the SOX9 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the SOX9 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using SOX9 antibody (clone PCRP-SOX9-1A2) . These results demonstrate the foremost specificity of the PCRP-SOX9-1A2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.