Recombinant PU.1 Antibody / SPI1

PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development.

Product Specifications

CAS Number

9000-83-3

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P17947

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 16-170 was used as the immunogen for the recombinant PU.1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RPU1/2146

Type

Recombinant

Applications

FACS, IF, WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant PU.1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant PU.1 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant PU.1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant PU.1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human lymph node with recombinant PU.1 antibody (clone rPU1/2146) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.