SOX10 Antibody
This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. Expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Often used melanoma markers, such as anti-HMB45 and anti-MelanA, are poorly expressed in desmoplastic melanomas while SOX10 is moderately-to-strongly expressed in desmoplastic melanomas.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL
UniProt
P56693
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 90-179 was used as the immunogen for the SOX10 antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
PCRP-SOX10-1D8
Applications
FACS, IF
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the SOX10 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This SOX10 antibody is available for research use only.
Storage Conditions
Aliquot the SOX10 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the SOX10 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
FACS staining of PFA-fixed human HeLa cells with SOX10 antibody (blue, clone PCRP-SOX10-1D8), and unstained cells (red) .
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