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Recombinant Keratin 5 Antibody / KRT5 / Cytokeratin 5

Cytokeratin 5/Keratin 5/KRT5 is specifically expressed in the basal layer of the epidermis with family member KRT14. Antibodies to KRT5 identify basal cells of squamous and glandular epithelia, myoepithelial, and mesothelium. Anti-cytokeratin 5 has been reported useful in the differential diagnosis of metastatic carcinoma in the pleura versus epithelioid mesothelioma. Almost all squamous cell carcinomas, half of transitional carcinomas, and many undifferentiated large cell carcinomas express.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P13647

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 316-590 was used as the immunogen for the recombinant Keratin 5 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

KRT5/6399R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant Keratin 5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant Keratin 5 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant Keratin 5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Keratin 5 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human prostate carcinoma tissue with recombinant Keratin 5 antibody (clone KRT5/6399R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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