ID1 Antibody
Members of the Id family of basic helix-loop-helix (bHLH) proteins include Id1, Id2, Id3 and Id4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The Id-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since Id1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA (order BSA-free format for coating), Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL
UniProt
P41134
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 5-150 was used as the immunogen for the ID1 antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
PCRP-ID1-2F11
Applications
ELISA, FACS, IF
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the ID1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This ID1 antibody is available for research use only.
Storage Conditions
Aliquot the ID1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the ID1 antibody should be determined by the researcher.
Location
Nucleus, Cytoplasm
Image Legend
Immunofluorescent staining of PFA-fixed human HeLa cells using ID1 antibody (green, clone PCRP-ID1-2F11) and phalloidin (red) .
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