Recombinant EpCAM Antibody
Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of Ep-CAM. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL
UniProt
P16422
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human Ep-CAM protein was used as the immunogen for the recombinant EpCAM antibody. The epitope is known to be between amino acids 27-59.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
REGP40/826
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the recombinant EpCAM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant EpCAM antibody is available for research use only.
Storage Conditions
Aliquot the recombinant EpCAM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant EpCAM antibody should be determined by the researcher.
Location
Cell surface, cytoplasmic
Image Legend
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Frequently Asked Questions
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