Recombinant Cytokeratin 20 Antibody / CK20
This MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20) . KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions. It has been detected in adenocarcinomas of the colon, stomach and biliary tract. Breast carcinomas are generally non-reactive.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL
UniProt
P35900
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 196-323 was used as the immunogen for the recombinant Cytokeratin 20 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG2a κ
Clone
RKRT20/6536
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the recombinant Cytokeratin 20 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant Cytokeratin 20 antibody is available for research use only.
Storage Conditions
Aliquot the recombinant Cytokeratin 20 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant Cytokeratin 20 antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
Western blot testing of human HT29 cell lysate with recombinant Cytokeratin 20 antibody. Predicted molecular weight ~46 kDa.
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