TIMP2 Antibody

It recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2) . It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A) . It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B) . TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P16035

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human TIMP2 protein was used as the immunogen for the TIMP2 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

TIMP2/4477

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the TIMP2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This TIMP2 antibody is available for research use only.

Storage Conditions

Aliquot the TIMP2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the TIMP2 antibody should be determined by the researcher.

Location

Secreted, Cytoplasm

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using TIMP2 antibody (clone TIMP2/4477) . These results demonstrate the foremost specificity of the TIMP2/4477 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.