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Recombinant MyoD1 Antibody

MyoD1, one of the MyoD family of myogenic helix-loop-helix transcription factors, combined with myogenin, plays a role in coordinating the myogenic differentiation pathway from the determination of mesodermal precursors into myoblasts, the differentiation of myoblasts into myotubes, and finally the maturation of myotubes into skeletal myofibers.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 2-4 µg/mL

UniProt

P15172

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 1-100 was used as the immunogen for the recombinant MyoD1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RMYOD1/6911

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the recombinant MyoD1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant MyoD1 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant MyoD1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the recombinant MyoD1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human rhabdomyosarcoma tissue with recombinant MyoD1 antibody (clone rMYOD1/6911) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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