KRT16 Antibody / Cytokeratin 16

Cytokeratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I Cytokeratins (Cytokeratins 9 to 23) and one type II Cytokeratins (keratins 1 to 8) . The cytokeratin proteins play a critical role in differentiation, as well as tissue specialization and function, to maintain the overall structural integrity of epithelial cells. Cytokeratins are also useful markers in identifying the origin of metastatic tumors. Cytokeratin 16 is expressed in benign stratified squamous epithelium and squamous cell carcinoma of the head and neck, as well as luminal cells of mammary gland and sweat ducts. It is absent in non-invasive breast carcinomas and normal breast tissue.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P08779

Host

Mouse

Reactivity

Human

Immunogen

A recombinant protein fragment from the C-terminal region was used as the immunogen for the KRT16 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

SPM264

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the KRT16 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This KRT16 antibody is available for research use only.

Storage Conditions

Aliquot the KRT16 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the KRT16 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human tonsil tissue with KRT16 antibody (clone SPM264) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.