Recombinant Wilms Tumor 1 Antibody / WT1

The WT1 gene located at chromosome 11p13 codes for a transcription factor, a DNA-binding nucleoprotein, that plays a role primarily in the development of genitourinary organs. There are at least eight isoforms ranging between 52 and 62kDa produced by a combination of alternative splicing and RNA editing. WT1 is synthesized and reside in the cytoplasm in an inactive form. When activated through phosphorylation it is translocated to the nucleus.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P19544

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 1-100 was used as the immunogen for the recombinant Wilms Tumor 1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

WT1/3477R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the recombinant Wilms Tumor 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant Wilms Tumor 1 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant Wilms Tumor 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the recombinant Wilms Tumor 1 antibody should be determined by the researcher.

Location

Nucleus and cytoplasm

Image Legend

IHC staining of FFPE human kidney tissue with recombinant Wilms Tumor 1 antibody (clone WT1/3477R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.