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MCAM Antibody / CD146 / MUC18

The human MCAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated Mel-CAM, MUC 18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. MCAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. MCAM is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: 2-4 µg/mL (order BSA-free format for coating)

UniProt

P43121

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human MCAM/CD146 protein was used as the immunogen for the MCAM antibody.

Clonality

Monoclonal

Isotype

IgG2c κ

Clone

MCAM/3179

Applications

ELISA

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the MCAM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This MCAM antibody is available for research use only.

Storage Conditions

Aliquot the MCAM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the MCAM antibody should be determined by the researcher.

Location

Cell Surface

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using MCAM antibody (clone MCAM/3179) . These results demonstrate the foremost specificity of the MCAM/3179 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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