ETS2 Antibody / C-ets-2

ETS2 (Protein C-ets-2) is a large glycoprotein that is secreted by Cajal-Retzius cells in the forebrain and by granule neurons in the cerebellum. ETS2 was shown to be mutated in reeler mice, a mutation that is associated with widespread disruption of laminated regions of the brain, leading to impaired motor coordination, tremors and ataxia. ETS2 protein expression is complex and changes throughout development. ETS2 appears to function upstream of Dab1, in a signaling pathway that controls cell positioning in the developing brain, and is also thought to be a direct effector of the neurotrophin BDNF.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA-free format for coating), Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL

UniProt

P15036

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human ETS2 protein was used as the immunogen for the ETS2 antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

PCRP-ETS2-1D9

Applications

ELISA, FACS, IF

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the ETS2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ETS2 antibody is available for research use only.

Storage Conditions

Aliquot the ETS2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the ETS2 antibody should be determined by the researcher.

Location

Nucleus, cytoplasm, plasma membrane

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using ETS2 antibody (clone PCRP-ETS2-1D9) . These results demonstrate the foremost specificity of the PCRP-ETS2-1D9 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.