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HA Tag Antibody

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA-free format for coating), Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate), Flow cytometry: 1-2ug/million cells, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Not Known

Host

Mouse

Immunogen

Amino acids 98-106 (YPYDVPDYA) were used as the immunogen for the HA Tag antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

HA/279

Applications

ELISA, IF, WB, IP, FACS, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the HA Tag antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This HA Tag antibody is available for research use only.

Storage Conditions

Aliquot the HA Tag antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the HA Tag antibody should be determined by the researcher.

Image Legend

SDS-PAGE analysis of purified, BSA-free HA Tag antibody (clone HA/279) as confirmation of integrity and purity.

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