CEA Antibody / Carcinoembryonic Antigen
This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+) . Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 1-2 µg/mL
UniProt
P06731
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human CEA protein was used as the immunogen for the CEA antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
C66/1292
Applications
FACS, IF
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the CEA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This CEA antibody is available for research use only.
Storage Conditions
Aliquot the CEA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the CEA antibody should be determined by the researcher.
Location
Cytoplasmic and luminal surface
Image Legend
SDS-PAGE analysis of purified, BSA-free CEA antibody (clone C66/1292) as confirmation of integrity and purity.
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