Recombinant Caldesmon Antibody / CAD / CALD1 (HMW)

Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kDa) is found in non- muscle tissue and cells.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL

UniProt

Q05682

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human CALD1 protein was used as the immunogen for the recombinant Caldesmon antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RCALD1/820

Type

Recombinant

Applications

WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant Caldesmon antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant Caldesmon antibody is available for research use only.

Storage Conditions

Aliquot the recombinant Caldesmon antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Caldesmon antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Western blot testing of human ovary tissue lysate using recombinant Caldesmon antibody (clone rCALD1/820) . Predicted molecular weight ~93 kDa, can be observed at 70-80 kDa (non muscle tissue) and 120-150 kDa (smooth muscle) .