AMACR / p504S Antibody (Prostate Cancer Marker)
AMACR, also known as p504S, is an enzyme that is involved in bile acid biosynthesis and -oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells) . Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium) . AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:50-1:100, Immunohistochemistry (FFPE) : 1:50-1:100
UniProt
Q9UHK6
Host
Rabbit
Reactivity
Human
Immunogen
A synthetic peptide from human AMACR protein was used as the immunogen for the AMACR antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Protein A affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the AMACR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This AMACR antibody is available for research use only.
Storage Conditions
Aliquot the AMACR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the AMACR antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Western blot testing of human kidney tissue lysate using AMACR antibody. Predicted molecular weight ~43 kDa.
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