CD63 Antibody / LAMP-3

CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: 1-5 µg/mL (order BSA-free format for coating), Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P08962

Host

Mouse

Reactivity

Human

Immunogen

Full-length human CD63 protein was used as the immunogen for the CD63 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

LAMP3/4949

Applications

ELISA, FACS, IF, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the CD63 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CD63 antibody is available for research use only.

Storage Conditions

Aliquot the CD63 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the CD63 antibody should be determined by the researcher.

Location

Cell surface, Cytoplasm

Image Legend

FACS staining of bead-bound exosomes derived from MCF-7 cells: Unstained exosomes (gray) and exosomes stained with CD63 antibody (orange, clone LAMP3/4949) .