Recombinant Lambda Light Chain Antibody

This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P01701 & P01842

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant full-length human IGL protein was used as the immunogen for the recombinant Lambda Light Chain antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

LLC/3774R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant Lambda Light Chain antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant Lambda Light Chain antibody is available for research use only.

Storage Conditions

Aliquot the recombinant Lambda Light Chain antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Lambda Light Chain antibody should be determined by the researcher.

Location

Cell Surface, cytoplasmic and secreted

Image Legend

IHC staining of FFPE human spleen tissue with recombinant Lambda Light Chain antibody (clone LLC/3774R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.