Biotin Antibody
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 0.5-1 µg/mL, Western blot: 0.5-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL
UniProt
Not Applicable
Host
Mouse
Reactivity
All Species Expected
Immunogen
Biotinylated sheep immunoglobulin was used as the immuongen for the Biotin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
BTN399
Applications
FACS, IF, WB, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Biotin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Biotin antibody is available for research use only.
Storage Conditions
Aliquot the Biotin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Biotin antibody should be determined by the researcher.
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