Biotin Antibody

It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 0.5-1 µg/mL, Western blot: 0.5-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL

UniProt

Not Applicable

Host

Mouse

Reactivity

All Species Expected

Immunogen

Biotinylated sheep immunoglobulin was used as the immuongen for the Biotin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

BTN399

Applications

FACS, IF, WB, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Biotin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Biotin antibody is available for research use only.

Storage Conditions

Aliquot the Biotin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Biotin antibody should be determined by the researcher.

Image Legend

IHC staining of endogenous biotin in FFPE human kidney tissue with Biotin antibody (clone BTN399) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.