Recombinant EpCAM Antibody / Cytoplasmic domain
Epithelial cellular adhesion molecule (Ep-CAM), also called epithelial specific antigen (ESA), is a 40-43kDa transmembrane epithelial glycoprotein. It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Product Specifications
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P16422
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 289-314 of human EpCAM was used as the immunogen for the recombinant EpCAM antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
EGP40/7035R
Type
Recombinant
Applications
IHC-P
Purity
Protein A affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the recombinant EpCAM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant EpCAM antibody is available for research use only.
Storage Conditions
Aliquot the recombinant EpCAM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant EpCAM antibody should be determined by the researcher.
Location
Cell surface, cytoplasmic
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