Recombinant EpCAM Antibody / Cytoplasmic domain

Epithelial cellular adhesion molecule (Ep-CAM), also called epithelial specific antigen (ESA), is a 40-43kDa transmembrane epithelial glycoprotein. It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P16422

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 289-314 of human EpCAM was used as the immunogen for the recombinant EpCAM antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

EGP40/7035R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant EpCAM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant EpCAM antibody is available for research use only.

Storage Conditions

Aliquot the recombinant EpCAM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant EpCAM antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic

Image Legend

IHC staining of FFPE human colon tissue with recombinant EpCAM antibody (clone EGP40/7035R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.