Muscle Actin Antibody (Skeletal/Cardiac/Smooth)
This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas) .
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-2 µg/mL
UniProt
P62736, P68032 & P68133
Host
Mouse
Reactivity
Human
Immunogen
Recombinant protein was used as the immunogen for the Muscle Actin antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RMSA/953
Type
Recombinant
Applications
WB, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Muscle Actin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Muscle Actin antibody is available for research use only.
Storage Conditions
Aliquot the Muscle Actin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Muscle Actin antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
Western blot testing of human skeletal muscle tissue lysate using Muscle Actin antibody (clone rMSA/953) . Predicted molecular weight ~45 kDa.
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