Bcl-2 Antibody
This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 lpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 lpha oncoprotein inhibits the programmed cell death (apoptosis) . In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 lpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P10415
Host
Mouse
Reactivity
Human
Immunogen
Amino acids 41-54 (GAAPAPGIFSSQPG) from the human protein were used as the immunogen for the Bcl-2 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SPM117
Applications
WB, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Bcl-2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Bcl-2 antibody is available for research use only.
Storage Conditions
Aliquot the Bcl-2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Bcl-2 antibody should be determined by the researcher.
Location
Cytoplasm, nuclear membrane
Image Legend
Western blot analysis of human MCF-7 cell lysate using Bcl-2 antibody (clone SPM117) .
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