IL1 beta Antibody / IL1B
Recognizes a protein of 17-31kDa, identified as interleukin 1beta. It shows no cross-reaction with interleukin 1alpha. Epitopes of MAb IL1B/463 and IL1B/6687 are different and provide a good pair for developing an ELISA. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE) . This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA (order BSA-free format for coating), Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P01584
Host
Mouse
Reactivity
Human
Immunogen
A recombinant protein fragment was used as the immunogen for the IL1 beta antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
IL1B/6687
Applications
ELISA, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the IL1 beta antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This IL1 beta antibody is available for research use only.
Storage Conditions
Aliquot the IL1 beta antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the IL1 beta antibody should be determined by the researcher.
Location
Cytoplasm, Extracellular (secreted)
Image Legend
Frequently Asked Questions
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