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Recombinant Human Nuclear Antigen Antibody

This antibody recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions.

Product Specifications

CAS Number

9000-83-3

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-3 µg/mL

UniProt

Not Known

Host

Mouse

Reactivity

Human

Immunogen

Nuclei of human myeloid leukemia biopsy cells were used as the immunogen for the recombinant Human Nuclear Antigen antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG2a κ

Clone

R235-1

Type

Recombinant

Applications

FACS, IF

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant Human Nuclear Antigen antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant Human Nuclear Antigen antibody is available for research use only.

Storage Conditions

Aliquot the recombinant Human Nuclear Antigen antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Human Nuclear Antigen antibody should be determined by the researcher.

Location

Nucleus

Image Legend

SDS-PAGE analysis of purified, BSA-free recombinant Human Nuclear Antigen antibody (r235-1) as confirmation of integrity and purity.

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