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CD44 Antibody / HCAM

The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s) . Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P16070

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human CD44 protein was used as the immunogen for the CD44 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG2a κ

Clone

RHCAM/6449

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the CD44 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CD44 antibody is available for research use only.

Storage Conditions

Aliquot the CD44 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the CD44 antibody should be determined by the researcher.

Location

Cell Surface

Image Legend

IHC staining of FFPE human esophagus tissue with CD44 antibody (clone rHCAM/6449) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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