Recombinant SDHB Antibody

Succinate dehydrogenase B (SDHB) is an iron-sulfur subunit of mitochondrial complex II, a key component of the citric acid cycle and the electron transport chain, a respiratory complex that catalyzes the oxidation of succinate in the mitochondrial membrane. Many cancers are generally positive for SDHB, including renal cell carcinomas and gastrointestinal stromal tumors. A subset of RCC and GIST tumors that are associated with SDH mutations, Carney-Stratakis Syndrome or Carney Triad exhibit a loss of SDHB expression.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P21912

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide specific to human SDHB was used as the immunogen for the recombinant SDHB antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

SDHB/6697R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant SDHB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant SDHB antibody is available for research use only.

Storage Conditions

Aliquot the recombinant SDHB antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant SDHB antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC staining of FFPE human heart tissue with recombinant SDHB antibody (clone SDHB/6697R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.