SDHB Antibody
Succinate dehydrogenase (SDH) is Complex II in the mitochondria, vital for mitochondrial electron transport, as well as Krebs cycle function.Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB) and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. The SDH complex functions as a tumor suppressor. Loss of any subunit proteins lead to destabilization of the complex and tumor formation.Antibody to SDHB is helpful in the identification of phaeochromocytomas, paragangliomas and GIST.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P21912
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 165-273 was used as the immunogen for the SDHB antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SDHB/2126
Applications
WB, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the SDHB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This SDHB antibody is available for research use only.
Storage Conditions
Aliquot the SDHB antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the SDHB antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Western blot testing of human 1) 293, 2) Jurkat and 3) HepG2 cell lysate using SDHB antibody (clone SDHB/2126) .
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