NKX3.1 Antibody

NKX3.1 is a prostate specific gene encoding a transcription factor that plays an important role in normal prostate development and carcinogenesis. It is a prostatic tumor suppressor gene located on chromosome 8p21.2, which frequently undergoes a loss of heterozygosity. NKX3.1 expression is highly restricted in prostate epithelial cells and therefore can be used as a diagnostic biomarker for prostate cancer and other metastatic lesions of prostatic origin. Furthermore, NKX3.1 shows better sensitivity than Prostate Specific Antigen (PSA) for identifying metastatic prostatic adenocarcinoma. This suggests that immunohistochemical staining of NKX3.1, along with other prostate-restricted markers, may be valuable for the definitive determination of prostatic origin in poorly differentiated metastatic carcinomas.

Product Specifications

CAS Number

9000-83-3

Specifications

ELISA (For coating order BSA-free format), Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q99801

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 92-224 was used as the immunogen for the NKX3.1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RNKX3.1/6620

Type

Recombinant

Applications

ELISA, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the NKX3.1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This NKX3.1 antibody is available for research use only.

Storage Conditions

Aliquot the NKX3.1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the NKX3.1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human prostate tissue with NKX3.1 antibody (clone rNKX3.1/6620) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.