CD3 epsilon Antibody
Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR) . Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
Product Specifications
CAS Number
9000-83-3
Specifications
ELISA: 2-4 µg/mL (for coating order BSA-free format)
UniProt
P07766
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 23-119 was used as the immunogen for the CD3 epsilon antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RC3e/2479
Type
Recombinant
Applications
ELISA
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the CD3 epsilon antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This CD3 epsilon antibody is available for research use only.
Storage Conditions
Aliquot the CD3 epsilon antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the CD3 epsilon antibody should be determined by the researcher.
Location
Cell Surface and cytoplasm
Image Legend
SDS-PAGE analysis of purified, BSA-free CD3 epsilon antibody (rC3e/2479) as confirmation of integrity and purity.
Documents
Frequently Asked Questions
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