Negative Control Antibody for Mouse Monoclonals

To serve as a true negative control for various procedures, this monoclonal IgG should be used under the identical conditions and at the same dilution as the rabbit monoclonal antibody being used for a positive reaction.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA; Flow cytometry; Immunofluorescence; Immunoprecipitation; Western blot; Immunohistochemistry: use at the same concentration as the primary antibody being controlled for

UniProt

Not Applicable

Host

Mouse

Immunogen

Recombinant full-length human IG1R protein was used as the immunogen for the Negative control antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

IGG1/453

Applications

ELISA, FACS, IF, IP, WB, IHC

Purity

Protein A affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Negative control antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Negative control antibody is available for research use only.

Storage Conditions

Aliquot the Negative control antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Negative control antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human heart tissue with Negative control antibody (clone IGG1/453) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.